14023 2310033P09Rik Status: ES cell colonies screened / QC no positives
| Gene | 2310033P09Rik |
| Chromosome | 11 |
| Deletion Start | 59,208,432 |
| Deletion End | 59,210,408 |
| Deletion Size | 1,977 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,208,811 | 59,208,829 | TGCTCGCAGTGGTTACAAG |
| TUP | 59,208,836 | 59,208,858 | AGGAAACAGCCCACCGGCCTGAG |
| TUR | 59,208,872 | 59,208,893 | GGACCTCGAACCCATATCTCAG |
| TDF | 59,209,485 | 59,209,506 | GGAGTCTCAGTAGCTGCCATAG |
| TDP | 59,209,508 | 59,209,528 | TGACCCAGCAGGGTGACGCTC |
| TDR | 59,209,548 | 59,209,567 | ACAAGCACCTCCCACCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCACCGAGATTCCTTCAC |
| SD | CAGCCTAACCTGTCCTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
