14024 2310036O22Rik Status: Cancelled
| Gene | 2310036O22Rik |
| Chromosome | 8 |
| Deletion Start | 85,026,902 |
| Deletion End | 85,030,072 |
| Deletion Size | 3,171 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,027,351 | 85,027,369 | CGGTCGCTTGCTGAGAATC |
| TUP | 85,027,371 | 85,027,392 | CGAGCCTGGATTCCGGACCAGA |
| TUR | 85,027,393 | 85,027,410 | TTCCCGCCCAGAAACACC |
| TDF | 85,029,508 | 85,029,528 | AACCCTTGCAGTCTTCTGTCC |
| TDP | 85,029,529 | 85,029,550 | CTTCACCCAGGTGGGCAAGCGC |
| TDR | 85,029,572 | 85,029,593 | TGGCTACTATTCCCGTCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | GCTCCAAACAAGATCCGTTC |
| SD | CCTGCCTACAGAACCTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
