14026 2410004B18Rik Status: ES cell colonies screened / QC no positives
| Gene | 2410004B18Rik |
| Chromosome | 3 |
| Deletion Start | 145,938,062 |
| Deletion End | 145,943,972 |
| Deletion Size | 5,911 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,938,809 | 145,938,830 | CAGAATGATGAGCAGGGAGGTG |
| TUP | 145,938,832 | 145,938,855 | ATGCGCTCGTATCCATCGCGGGAA |
| TUR | 145,938,886 | 145,938,907 | CCTTTGGAGGCTGTCGAGTAAG |
| TDF | 145,943,417 | 145,943,434 | ACTCGGCACGGCTAGAAG |
| TDP | 145,943,436 | 145,943,461 | TGCATGCTTTGTAGAAGGAGCAGTGC |
| TDR | 145,943,503 | 145,943,523 | CGCAAACTCTCCAGCACAAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGGACTTACATGCACCTCG |
| SD | AAACGTCACAGGGCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
