14038 3830406C13Rik Status: ES cell colonies screened / QC no positives
| Gene | 3830406C13Rik |
| Chromosome | 14 |
| Deletion Start | 12,285,419 |
| Deletion End | 12,301,320 |
| Deletion Size | 15,902 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,285,990 | 12,286,008 | GCCAGGCAGCTCAGAAGAG |
| TUP | 12,286,011 | 12,286,035 | TGAGAGGGCACCAGAATCCCTGGAG |
| TUR | 12,286,055 | 12,286,077 | AGCATCCACATAAAGCAGCAATC |
| TDF | 12,300,352 | 12,300,374 | GATCCCAGCACCCACTTAAAGAG |
| TDP | 12,300,375 | 12,300,398 | CCAGACATGGCCCAAATGCCGTAA |
| TDR | 12,300,421 | 12,300,442 | TGGCAATAGTAGTCCTCCTGTG |
PCR Primers
| Primer | Sequence |
| SU | ATGTCCATTAGTCTTAGTGCC |
| SD | TCTGTAGGAAGCACACAACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
