14039 Ndufaf3 Status: ES cell colonies screened / QC no positives
| Gene | Ndufaf3 |
| Chromosome | 9 |
| Deletion Start | 108,566,924 |
| Deletion End | 108,565,859 |
| Deletion Size | 1,066 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,566,671 | 108,566,690 | CGCATCTCACTGCTGCAAAG |
| TUP | 108,566,644 | 108,566,668 | AGTTCCCTCAGGCCGTGTACATCGA |
| TUR | 108,566,586 | 108,566,603 | CACGCAAGGGCCAAACAC |
| TDF | 108,566,234 | 108,566,252 | TGTGGTGGGCACTGGAAAC |
| TDP | 108,566,211 | 108,566,233 | AAGACTGAGCGGCTGCACCCTCA |
| TDR | 108,566,188 | 108,566,206 | GCTGCCTCATGGCCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGGTGTTCATAACAGG |
| SD | TCCCTACAGGTACGAGAAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
