14041 4930572J05Rik Status: Cancelled
| Gene | 4930572J05Rik |
| Chromosome | 15 |
| Deletion Start | 74,721,298 |
| Deletion End | 74,723,644 |
| Deletion Size | 2,347 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,721,734 | 74,721,753 | CCTTGCTGCGTTTCCGACAG |
| TUP | 74,721,765 | 74,721,790 | CACCTCGCCGGATCGAGTAGTACAGC |
| TUR | 74,721,798 | 74,721,816 | GCTGCTCACCCTCCGTTTG |
| TDF | 74,722,848 | 74,722,869 | GGGTAGGGTTCTGTAAGCTGAG |
| TDP | 74,722,871 | 74,722,893 | AACTGTTCTGAGGCCAGGTGCCA |
| TDR | 74,722,918 | 74,722,936 | AGGCTCTATCTGGGCACAC |
PCR Primers
| Primer | Sequence |
| SU | GACTGGATCACTAGCGGGAG |
| SD | GTTCTGGTCTTGCTTCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
