14045 Ankrd33b Status: Cancelled
| Gene | Ankrd33b |
| Chromosome | 15 |
| Deletion Start | 31,367,389 |
| Deletion End | 31,367,070 |
| Deletion Size | 320 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,367,296 | 31,367,314 | AGCGAGCAGACAGGAGAAG |
| TUP | 31,367,269 | 31,367,294 | CCCAGCGGACTACGAGGAGTACGAAG |
| TUR | 31,367,247 | 31,367,267 | GTGTCCGGCAGACTAGAGAAG |
| TDF | 31,367,181 | 31,367,201 | GGAAGAGTACGGCTCGGTGAG |
| TDP | 31,367,153 | 31,367,171 | CGTCCCGGAGGGCGTCCCT |
| TDR | 31,367,091 | 31,367,108 | ACTAGCGCCCGCAGCAAC |
PCR Primers
| Primer | Sequence |
| SU | TTGGAGAGGATCTCTGATGC |
| SD | TCAGATGTCACCACATTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
