| Gene | 6430531B16Rik |
| Chromosome | 7 |
| Deletion Start | 139,978,679 |
| Deletion End | 139,972,369 |
| Deletion Size | 6,311 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,978,163 | 139,978,181 | GACCAGGCAAGCCAAGTTC |
| TUP | 139,978,139 | 139,978,161 | CCTCGCATTCCCACAGTGCTGGT |
| TUR | 139,978,113 | 139,978,132 | GGCGGAAGTGCTTCACAATC |
| TDF | 139,972,529 | 139,972,551 | GATCTGGGAGCTGATGAGAACTG |
| TDP | 139,972,505 | 139,972,527 | ACCCGAGGAGCATCAGAGCTGGA |
| TDR | 139,972,467 | 139,972,486 | TCAGGATCGAGGTCTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | CGTCATCTCCGGATTGAAAC |
| SD | GGTCTCAGGTTCAGGTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14048A-A5 | VGB6 | C57BL/6NTac | |
| 14048A-A7 | VGB6 | C57BL/6NTac | |
| 14048A-C11 | VGB6 | C57BL/6NTac | |
| 14048A-D7 | VGB6 | C57BL/6NTac | |
| 14048A-F4 | VGB6 | C57BL/6NTac | |
| 14048A-G4 | VGB6 | C57BL/6NTac | |
