14049 6430571L13Rik Status: Cancelled
| Gene | 6430571L13Rik |
| Chromosome | 9 |
| Deletion Start | 107,342,353 |
| Deletion End | 107,348,049 |
| Deletion Size | 5,697 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,342,908 | 107,342,927 | GGCAGTGAAGCAGAGGAGTG |
| TUP | 107,342,928 | 107,342,953 | TGGGAGTTTCAATAGCAGCAGAGGCC |
| TUR | 107,342,961 | 107,342,982 | GCATCCCACATAATAGCCCAAC |
| TDF | 107,347,536 | 107,347,555 | GAGCCACTGAGCAACCAAAC |
| TDP | 107,347,559 | 107,347,584 | TCGCTATCATTCTCCCAAGTCCTCCA |
| TDR | 107,347,611 | 107,347,634 | CAAACACAAGGATGGATCAAGGAC |
PCR Primers
| Primer | Sequence |
| SU | TCCCTACAGAAGCCCATGTC |
| SD | AAAGGTATTGCCCTGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
