| Gene | A230050P20Rik |
| Chromosome | 9 |
| Deletion Start | 20,868,919 |
| Deletion End | 20,873,869 |
| Deletion Size | 4,951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,869,552 | 20,869,570 | CCGCCTGGAACTCCTACAC |
| TUP | 20,869,582 | 20,869,608 | CCCTCCCATGTTCTCCCACTAGGTGTC |
| TUR | 20,869,614 | 20,869,632 | CAGCTCCTGTCCGTCTTTC |
| TDF | 20,872,871 | 20,872,890 | TCACGGTGTCGCAAGTGTAG |
| TDP | 20,872,893 | 20,872,919 | AGCGTTACGAACCAGTGCCACTTGACA |
| TDR | 20,872,937 | 20,872,956 | CACTTTGGGCAGTGGAACTC |
PCR Primers
| Primer | Sequence |
| SU | ACATGTGACCGACTGCAGAG |
| SD | TTTGCACAAAGAAGTGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14054A-A6 | VGB6 | C57BL/6NTac | |
| 14054A-E12 | VGB6 | C57BL/6NTac | |
