14065 Agrp Status: Cancelled
| Gene | Agrp |
| Chromosome | 8 |
| Deletion Start | 105,567,486 |
| Deletion End | 105,566,683 |
| Deletion Size | 804 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,567,238 | 105,567,259 | CTAGGGAGGTGGGTACTTGGTG |
| TUP | 105,567,216 | 105,567,237 | CATGGTGGGTGTGGCCCTCACA |
| TUR | 105,567,179 | 105,567,202 | TCTTGAGGCCATTCAGACTTAGAC |
| TDF | 105,566,889 | 105,566,906 | TCCGCGTCGCTGTGTAAG |
| TDP | 105,566,865 | 105,566,887 | CTGCACGAGTCCTGCTTGGGACA |
| TDR | 105,566,807 | 105,566,831 | AAAGGCATTGAAGAAGCGGCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGACCCATCCCTAGGTTCTG |
| SD | CTTAGGCTGTCGAGGCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
