14066 Apoe Status: ES cell colonies screened / QC no positives
| Gene | Apoe |
| Chromosome | 7 |
| Deletion Start | 19,698,227 |
| Deletion End | 19,696,357 |
| Deletion Size | 1,871 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,697,657 | 19,697,678 | CCTGACCCAGCCTTAAACTTAC |
| TUP | 19,697,631 | 19,697,652 | CACAGGATGCCTAGCCGAGGGA |
| TUR | 19,697,581 | 19,697,600 | TCCCAGGGTTGGTTGCTTTG |
| TDF | 19,696,440 | 19,696,459 | GGGCAAACCTGATGGAGAAG |
| TDP | 19,696,414 | 19,696,438 | TACAGGCCTCTGTGGCTACCAACCC |
| TDR | 19,696,360 | 19,696,379 | GCAGGACAGGAGAAGGATAC |
PCR Primers
| Primer | Sequence |
| SU | GGACTTAGCCGGGAAGAAAG |
| SD | AGCCCATGCCTAGAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
