14070 Fcer1g Status: Cancelled
| Gene | Fcer1g |
| Chromosome | 1 |
| Deletion Start | 171,234,242 |
| Deletion End | 171,229,864 |
| Deletion Size | 4,379 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 171,233,492 | 171,233,512 | AGCCCAAGTTAGCCTTGAAAC |
| TUP | 171,233,457 | 171,233,484 | TTAGCTGAGGGTAACCTTGGTTTCCTGG |
| TUR | 171,233,435 | 171,233,454 | TTGGAAGGAGAAGGCAGGAG |
| TDF | 171,230,176 | 171,230,195 | GGCAGCTACCATTGCTCTTG |
| TDP | 171,230,149 | 171,230,174 | CCCAGATACTCCCTGGGTAGGAAGGA |
| TDR | 171,230,063 | 171,230,083 | TCACCGTGTAGACAGCATCTG |
PCR Primers
| Primer | Sequence |
| SU | GGTGGGTGGTTAAATATGGC |
| SD | CAGCATTCATACGCCAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
