14078 Il23a Status: Cancelled
| Gene | Il23a |
| Chromosome | 10 |
| Deletion Start | 128,297,969 |
| Deletion End | 128,296,754 |
| Deletion Size | 1,216 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,297,657 | 128,297,677 | GGGCTTCTGGAAGAGTTGTGG |
| TUP | 128,297,626 | 128,297,648 | TTGAGCGGAATGCAAAGCGGTCA |
| TUR | 128,297,595 | 128,297,615 | TAATGGAGGGAGTGGGAACAG |
| TDF | 128,297,130 | 128,297,152 | GAGCCTGCTCTACTCCCTGATAG |
| TDP | 128,297,103 | 128,297,128 | CCCATGGAGCAACTTCACACCTCCCT |
| TDR | 128,297,077 | 128,297,095 | CCTGGAGGAGTTGGCTGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGTCCATTACTTCCCTGCC |
| SD | GAACTGAGGGACAGAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
