| Gene | Lgr5 |
| Chromosome | 10 |
| Deletion Start | 115,587,490 |
| Deletion End | 115,587,288 |
| Deletion Size | 203 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,587,462 | 115,587,480 | GCGTCCACATGCTCCTGTC |
| TUP | 115,587,441 | 115,587,461 | CTTGCTGGCGCTGCTGCAGTT |
| TUR | 115,587,399 | 115,587,417 | CGCGGTATCGCATCTGGTC |
| TDF | 115,587,356 | 115,587,375 | GTGAGCTGGATGGCAGGATG |
| TDP | 115,587,330 | 115,587,352 | CTCAGGGTGGACTGCTCGGACCT |
| TDR | 115,587,288 | 115,587,308 | GAGGTGAAGACGCTGAGGTTG |
PCR Primers
| Primer | Sequence |
| SU | GATGGTGGGAGGGAGGAG |
| SD | CACACAAATGCGCACATTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14080A-D7 | VGB6 | C57BL/6NTac | |
| 14080A-D11 | VGB6 | C57BL/6NTac | |
| 14080A-F12 | VGB6 | C57BL/6NTac | |
| 14080A-F11 | VGB6 | C57BL/6NTac | |
| 14080A-G4 | VGB6 | C57BL/6NTac | |
