14081 Lrrtm3 Status: ES cell colonies screened / QC no positives
| Gene | Lrrtm3 |
| Chromosome | 10 |
| Deletion Start | 64,089,710 |
| Deletion End | 64,087,855 |
| Deletion Size | 1,856 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,088,875 | 64,088,895 | CCGCTCCAACTCACTGAGAAC |
| TUP | 64,088,851 | 64,088,874 | CATCCCGGTGCGGATCTTCCAAGA |
| TUR | 64,088,813 | 64,088,834 | GGTTGTATCCCAGGTCCAGAAG |
| TDF | 64,088,139 | 64,088,156 | GTGGACACGGAGCACATC |
| TDP | 64,088,114 | 64,088,138 | TCCTTCCATAAGATCATCGCGGGCA |
| TDR | 64,088,054 | 64,088,075 | GCTTCCAGGACACGTACATCAC |
PCR Primers
| Primer | Sequence |
| SU | AGATGTGGCAGCACAGGTAG |
| SD | AAAGAGTCTTAGACACTAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
