14086 Scgb3a1 Status: Cancelled
| Gene | Scgb3a1 |
| Chromosome | 11 |
| Deletion Start | 49,663,664 |
| Deletion End | 49,665,067 |
| Deletion Size | 1,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,664,049 | 49,664,067 | GGCCAGCCCTGGATTGATG |
| TUP | 49,664,071 | 49,664,095 | CCCATCCCATCTGCTCTCAACCTGG |
| TUR | 49,664,106 | 49,664,125 | ACTGTGCCTTGCTGATTGTG |
| TDF | 49,664,577 | 49,664,598 | AGGCTGTAGGAGCTGTGAAGTC |
| TDP | 49,664,601 | 49,664,628 | TGCTGGTAACATGGGCATCTTCCTTTCG |
| TDR | 49,664,631 | 49,664,651 | AACCACCCAGGAGGAAGTAAC |
PCR Primers
| Primer | Sequence |
| SU | TATCAAGGTGTAGGCCTGGG |
| SD | CTAGCGTCTTCCACAAAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
