| Gene | Ddx5 |
| Chromosome | 11 |
| Deletion Start | 106,788,313 |
| Deletion End | 106,781,772 |
| Deletion Size | 6,542 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,787,564 | 106,787,582 | CCTTGGTTTGCAGCCGTTC |
| TUP | 106,787,539 | 106,787,562 | CGGCGTTTCCCTAGACTCGGCTCG |
| TUR | 106,787,466 | 106,787,483 | GGCCGCAAAGGCAATGTG |
| TDF | 106,782,440 | 106,782,464 | GGATCTTCGTAAGAGATGTTTCTGG |
| TDP | 106,782,419 | 106,782,439 | TAGGTGGCAGTTACCTCTTGT |
| TDR | 106,782,354 | 106,782,381 | AAGTGCTTTTAGATTCTTTAGGTTTCAA |
PCR Primers
| Primer | Sequence |
| SU | GAAGCGAGTAATAAACGGGC |
| SD | AAGGGCCTTCTGAGTTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14088A-C7 | VGB6 | C57BL/6NTac | |
| 14088A-D10 | VGB6 | C57BL/6NTac | |
| 14088A-H11 | VGB6 | C57BL/6NTac | |
| 14088A-F3 | VGB6 | C57BL/6NTac | |
| 14088A-E8 | VGB6 | C57BL/6NTac | |
| 14088A-F10 | VGB6 | C57BL/6NTac | |
