14097 Trpa1 Status: ES cell colonies screened / QC one positive
| Gene | Trpa1 |
| Chromosome | 1 |
| Deletion Start | 14,918,832 |
| Deletion End | 14,889,400 |
| Deletion Size | 29,433 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,918,619 | 14,918,643 | TGCTGCTTTCACACTCAAGTTTATG |
| TUP | 14,918,593 | 14,918,618 | AAGCACGGAACATGGAAATGGCAGGA |
| TUR | 14,918,572 | 14,918,592 | GCTGTGTACCCTGATACTGTC |
| TDF | 14,889,753 | 14,889,776 | GCTTACAATCACTGCTGCTCTTTC |
| TDP | 14,889,714 | 14,889,739 | TTGATTCTCAGTACCGTCATGGTGGC |
| TDR | 14,889,670 | 14,889,694 | GACTACACTGAATCCACTTGAACTG |
PCR Primers
| Primer | Sequence |
| SU | CTCTTGTCCACTCCTGCCTC |
| SD | GGCTATCATAATGCAGAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
