| Gene | Sik1 |
| Chromosome | 17 |
| Deletion Start | 31,855,046 |
| Deletion End | 31,846,226 |
| Deletion Size | 8,821 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,854,194 | 31,854,214 | TTATCAGGTGAGCCGAAGGAC |
| TUP | 31,854,172 | 31,854,192 | CTCGCCGCACCGTGAGCACAT |
| TUR | 31,854,120 | 31,854,140 | GACTACCTTCAGGGACCCAAG |
| TDF | 31,846,830 | 31,846,849 | CTCCTGTTGAGCCTGACATC |
| TDP | 31,846,805 | 31,846,829 | TCTGAGTTGCCACAACAGCCAGGAT |
| TDR | 31,846,776 | 31,846,795 | TGACACATCCCTGAGCCATC |
PCR Primers
| Primer | Sequence |
| SU | TAGAGGAGTGTGTGCGTGG |
| SD | GCGTTTCAGAAGTGGAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14108A-D8 | VGB6 | C57BL/6NTac | |
| 14108A-D9 | VGB6 | C57BL/6NTac | |
| 14108A-H8 | VGB6 | C57BL/6NTac | |
