14115 Casc1 Status: ES cell colonies screened / QC positives
| Gene | Casc1 |
| Chromosome | 6 |
| Deletion Start | 145,210,806 |
| Deletion End | 145,175,033 |
| Deletion Size | 35,774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,210,776 | 145,210,798 | GGAAAGTGAGGGAGACAGGAAAG |
| TUP | 145,210,749 | 145,210,772 | CAAGTGCGGATTCCACTTCAGCGC |
| TUR | 145,210,691 | 145,210,711 | ATCTGGATCTCGCCACCTCTG |
| TDF | 145,175,119 | 145,175,137 | TGGAGAGGGTCAGGCATTC |
| TDP | 145,175,091 | 145,175,117 | AACTGCCAGTTCATCGACTCAGTGTGC |
| TDR | 145,175,060 | 145,175,077 | AGCTGAGGACGCGGATAG |
PCR Primers
| Primer | Sequence |
| SU | TACAGGCAGCAGTGTATGGG |
| SD | ATGGCCATTTACCAGACTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
