14185 Unc13c Status: ES cell colonies screened / QC no positives
| Gene | Unc13c |
| Chromosome | 9 |
| Deletion Start | 73,933,564 |
| Deletion End | 73,930,601 |
| Deletion Size | 2,964 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,933,025 | 73,933,044 | TGGGTGCTCTACGGAAACTG |
| TUP | 73,932,992 | 73,933,020 | AATGGAAGAAGAGCCAAGAGTGCGTCTCC |
| TUR | 73,932,971 | 73,932,990 | GGTGCTCAACTCCGAGTCTG |
| TDF | 73,930,977 | 73,930,998 | CCGAGGAAGAGGAGGACTACTG |
| TDP | 73,930,952 | 73,930,976 | TGAACCAGTGGCTGACAGTGAGACA |
| TDR | 73,930,920 | 73,930,939 | GCTAGGACCTGCTCCATCAC |
PCR Primers
| Primer | Sequence |
| SU | CTTGGGAAATGTAAGAAATGGG |
| SD | GCTAGGACAACAATCTTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
