14193 Stxbp5 Status: ES cell colonies screened / QC no positives
| Gene | Stxbp5 |
| Chromosome | 10 |
| Deletion Start | 9,900,680 |
| Deletion End | 9,866,617 |
| Deletion Size | 34,064 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,899,959 | 9,899,983 | GATGATTGCCACAAGGTCTTGTATG |
| TUP | 9,899,921 | 9,899,950 | AAAGAATGATGCAGCGGATTAATTCCCAGC |
| TUR | 9,899,870 | 9,899,891 | GGAAGCAGGCGATTCTGGATAG |
| TDF | 9,866,678 | 9,866,697 | CAGCTTTGGTCGTCCAGGAG |
| TDP | 9,866,652 | 9,866,677 | TGGAATGTTATTGCCAGCACGACAGC |
| TDR | 9,866,621 | 9,866,642 | TCAGGAACTGGAGTTGGATCAC |
PCR Primers
| Primer | Sequence |
| SU | CTCTACCGAGCGTTGAGGG |
| SD | AGATTGTTTAATGCTATGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
