14237 Maf Status: ES cell colonies screened / QC no positives
| Gene | Maf |
| Chromosome | 8 |
| Deletion Start | 115,706,860 |
| Deletion End | 115,705,729 |
| Deletion Size | 1,132 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,706,579 | 115,706,601 | TGGAAGACTACTACTGGATGACC |
| TUP | 115,706,555 | 115,706,576 | CTACCCGCAGCAGCTCAACCCG |
| TUR | 115,706,475 | 115,706,494 | CCATCGAAGCCACCCTGGAG |
| TDF | 115,705,947 | 115,705,966 | CAGTCCTGCCGCTTCAAGAG |
| TDP | 115,705,922 | 115,705,944 | TGCAGCAGAGACACGTCCTGGAG |
| TDR | 115,705,875 | 115,705,894 | TCCTGCTTGAGGTGGTCTAC |
PCR Primers
| Primer | Sequence |
| SU | GTGTGCACGTTCGAGCTTTC |
| SD | TTCAAAGGTGAGCTATGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
