14256   Grxcr1     Status: ES cell colonies screened / QC positives

GeneGrxcr1
Chromosome5
Deletion Start68,423,148
Deletion End68,423,522
Deletion Size375
Genome Build37
MGI IDMGI:3577767
NCBI Gene ID433899
BAC ID237E11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFAAGGGAGACAAAGCCAGAAAGTG
TUPAGGCCACGGAAAGTCCGGTTTCGA
TURCCTGGTGCTTGTCCATCTTCATAG
TDFGAACAGGAGAAAGATCAGGATAACC
TDPTGCTGGTATTAGCCAGGACAGCCA
TDRCTTCTTGTGCCAAAAGCCTTC

PCR Primers

PrimerSequence
SUAGGCCACTTTCTTGCACTTG
SDACACTACAATCTGCTGTCTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14256A-B6VGB6C57BL/6NTac
14256A-H12VGB6C57BL/6NTac