14286 Cep290 Status: Cancelled
| Gene | Cep290 |
| Chromosome | 10 |
| Deletion Start | 100,488,310 |
| Deletion End | 100,518,870 |
| Deletion Size | 30,561 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,489,193 | 100,489,216 | TGTCTCTGTGTGTTAGTCATCGTA |
| TUP | 100,489,217 | 100,489,238 | CACACTACAGACTGAGATAGCA |
| TUR | 100,489,241 | 100,489,261 | GGGAATGGAAACTGTGTGGAA |
| TDF | 100,518,556 | 100,518,576 | CGTAAGTGTAGCTCCGAGCAC |
| TDP | 100,518,577 | 100,518,598 | CCTTCTGGCCGCAGGGACACAT |
| TDR | 100,518,611 | 100,518,633 | CACAGCGACATTACTGAGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCAAACATCTTGGGAGTTGG |
| SD | CAGTGTTTTCAGTAGAGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
