14292 Arf1 Status: ES cell colonies screened / QC no positives
| Gene | Arf1 |
| Chromosome | 11 |
| Deletion Start | 59,213,484 |
| Deletion End | 59,212,451 |
| Deletion Size | 1,034 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,213,148 | 59,213,168 | GCGCCACTACTTCCAGAACAC |
| TUP | 59,213,114 | 59,213,141 | TAAGCAGCCAGTCCTTGTCCTAGATTCC |
| TUR | 59,213,061 | 59,213,080 | GCAAAGACCACAAGGCTCAG |
| TDF | 59,212,836 | 59,212,856 | CGAGATGCTGTTCTCTTGGTG |
| TDP | 59,212,810 | 59,212,833 | TGCCAACAAGCAGGTAGGCACCAG |
| TDR | 59,212,761 | 59,212,783 | CCCTCCTCTTTCTTCCCTGATAC |
PCR Primers
| Primer | Sequence |
| SU | TTGTGGTATCTGCGTCTTCC |
| SD | TCCGTTCTGTGTGTTCAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
