14292 Arf1 Status: ES cell colonies screened / QC no positives
Gene | Arf1 |
Chromosome | 11 |
Deletion Start | 59,213,484 |
Deletion End | 59,212,451 |
Deletion Size | 1,034 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 59,213,148 | 59,213,168 | GCGCCACTACTTCCAGAACAC |
TUP | 59,213,114 | 59,213,141 | TAAGCAGCCAGTCCTTGTCCTAGATTCC |
TUR | 59,213,061 | 59,213,080 | GCAAAGACCACAAGGCTCAG |
TDF | 59,212,836 | 59,212,856 | CGAGATGCTGTTCTCTTGGTG |
TDP | 59,212,810 | 59,212,833 | TGCCAACAAGCAGGTAGGCACCAG |
TDR | 59,212,761 | 59,212,783 | CCCTCCTCTTTCTTCCCTGATAC |
PCR Primers
Primer | Sequence |
SU | TTGTGGTATCTGCGTCTTCC |
SD | TCCGTTCTGTGTGTTCAAGTG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 108 bp | + | + | - |
TDF | TDR | 96 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |