14293 Arap2 Status: ES cell colonies screened / QC no positives
| Gene | Arap2 |
| Chromosome | 5 |
| Deletion Start | 62,749,671 |
| Deletion End | 62,714,737 |
| Deletion Size | 34,935 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,749,373 | 62,749,395 | CACAGTGATCCATCTTCCAGCAC |
| TUP | 62,749,349 | 62,749,372 | CCACACCGGCATAGAGTGTTCCGA |
| TUR | 62,749,322 | 62,749,344 | GGGCCAGGTCTATGGACAGTAAT |
| TDF | 62,715,049 | 62,715,066 | TTGTGCAGGGACCCTCAC |
| TDP | 62,715,023 | 62,715,045 | TGCATGTGAAGGCAACGTCGACA |
| TDR | 62,714,919 | 62,714,942 | GGCTGTAACAACTTTCAGGTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CCAGGCTTTGCTAATTGAAAC |
| SD | ATCATGGAGAGTGATTACGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
