14295 Ankrd13d Status: ES cell colonies screened / QC no positives
| Gene | Ankrd13d |
| Chromosome | 19 |
| Deletion Start | 4,282,967 |
| Deletion End | 4,270,335 |
| Deletion Size | 12,633 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,282,491 | 4,282,510 | TGGATAGCAGAGGGCAGAAG |
| TUP | 4,282,463 | 4,282,488 | ATGAGCAGTTCCTTCCCACTGGCCCT |
| TUR | 4,282,420 | 4,282,440 | TGGCCTATGTGGACTGGAAAG |
| TDF | 4,270,945 | 4,270,964 | TGGGATGCAAGTGGAAGGTG |
| TDP | 4,270,920 | 4,270,942 | TCATGGCCTCTACAGCCACAGGC |
| TDR | 4,270,890 | 4,270,908 | AGCAGCCAGAGCCCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGAAGTTCCGGCTGTTCC |
| SD | AATAGGAAGACCCAGCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
