14297 Adra1b Status: ES cell colonies screened / QC no positives
| Gene | Adra1b |
| Chromosome | 11 |
| Deletion Start | 43,836,085 |
| Deletion End | 43,835,144 |
| Deletion Size | 942 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,835,713 | 43,835,730 | TCTGGGCAGCGGTTGATG |
| TUP | 43,835,691 | 43,835,712 | TCCTGTGTTGCACGGCCTCCAT |
| TUR | 43,835,670 | 43,835,690 | GAGATGGCACATAGGCTCAGG |
| TDF | 43,835,561 | 43,835,579 | GGTCTTGTCCACGGTCATC |
| TDP | 43,835,538 | 43,835,560 | TCCATCGGGCCTCTCCTTGGATG |
| TDR | 43,835,518 | 43,835,537 | CATTGGGCGCAGGTTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCATTGAAAGCAGACCCTCC |
| SD | CAGTGACTGAGTCATTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
