14301   Abca17     Status: ES cell colonies screened / QC no positives

GeneAbca17
Chromosome17
Deletion Start24,347,249
Deletion End24,313,197
Deletion Size34,053
Genome Build38
MGI IDMGI:3625331
NCBI Gene ID381072
BAC ID262I14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,347,03924,347,057CTCTGGGTCACGGCGATTG
TUP24,347,00624,347,029CTTGAGGCAGCTGGTGCTGTCACA
TUR24,346,97424,346,993GGCTCTCAGACTGGCCTTTG
TDF24,313,31824,313,339TGCTCTGACTTTCCTACAGGTG
TDP24,313,28724,313,311TCGGGAAGGAGTAAACGTACAGCCA
TDR24,313,22524,313,245TCCGTTGTGTCCCAGAAGAAC

PCR Primers

PrimerSequence
SUAAAGAGCGAAAGAAATCTGCC
SDGTAGGCTGATGGAAAGACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR84 bp++-
TDFTDR115 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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