| Gene | Lgals8 |
| Chromosome | 13 |
| Deletion Start | 12,459,212 |
| Deletion End | 12,440,786 |
| Deletion Size | 18,427 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,458,918 | 12,458,941 | TCTACCATTTGGGTCTGGAAGTTG |
| TUP | 12,458,889 | 12,458,917 | AACTCAGGTGATCAGATTTGGCAGGAGGC |
| TUR | 12,458,866 | 12,458,887 | ACCATAGCTCAGCAGGTAAAGG |
| TDF | 12,441,393 | 12,441,414 | TGTCCGTCGATGTGGTTAAGAC |
| TDP | 12,441,368 | 12,441,392 | CTCTAGCACTGAGGCACGGTGTTCA |
| TDR | 12,441,296 | 12,441,317 | GGGAACTAACAGAAAGCTGCTG |
PCR Primers
| Primer | Sequence |
| SU | GACTATATGGTAGGACTTTGG |
| SD | GGCCAGAAACTGGTACGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14305A-F8 | VGB6 | C57BL/6NTac | |
| 14305C-B4 | VGB6 | C57BL/6NTac | |
