14305   Lgals8     Status: ES cell clone microinjected

GeneLgals8
Chromosome13
Deletion Start12,459,212
Deletion End12,440,786
Deletion Size18,427
Genome Build38
MGI IDMGI:1928481
NCBI Gene ID0
BAC ID383H12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF12,458,91812,458,941TCTACCATTTGGGTCTGGAAGTTG
TUP12,458,88912,458,917AACTCAGGTGATCAGATTTGGCAGGAGGC
TUR12,458,86612,458,887ACCATAGCTCAGCAGGTAAAGG
TDF12,441,39312,441,414TGTCCGTCGATGTGGTTAAGAC
TDP12,441,36812,441,392CTCTAGCACTGAGGCACGGTGTTCA
TDR12,441,29612,441,317GGGAACTAACAGAAAGCTGCTG

PCR Primers

PrimerSequence
SUGACTATATGGTAGGACTTTGG
SDGGCCAGAAACTGGTACGAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR76 bp++-
TDFTDR119 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14305A-F8VGB6C57BL/6NTac
14305C-B4VGB6C57BL/6NTac