| Gene | Ccr7 |
| Chromosome | 11 |
| Deletion Start | 99,154,995 |
| Deletion End | 99,144,892 |
| Deletion Size | 10,104 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,154,690 | 99,154,710 | GCAATGAACAGCCTGGTAGAC |
| TUP | 99,154,663 | 99,154,689 | TGACCTTGATGCCTGGATAGAACACGT |
| TUR | 99,154,620 | 99,154,638 | CCCAGAGGTGCCAGAGAAC |
| TDF | 99,145,811 | 99,145,832 | GGCTCAAGACCATGACGGATAC |
| TDP | 99,145,788 | 99,145,809 | TACCTGCTCAACCTGGCCGTGG |
| TDR | 99,145,742 | 99,145,759 | TCGCTGTAGGCCCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCACTCACAGATGTAAGCAG |
| SD | GCTCTGTCCTTGAATCTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14306A-C6 | VGB6 | C57BL/6NTac | |
| 14306A-A5 | VGB6 | C57BL/6NTac | |
