14307 Trpv4 Status: ES cell colonies screened / QC no positives
| Gene | Trpv4 |
| Chromosome | 5 |
| Deletion Start | 114,644,896 |
| Deletion End | 114,622,598 |
| Deletion Size | 22,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,644,561 | 114,644,582 | CTTGTTCGACTACGGCACTTAC |
| TUP | 114,644,525 | 114,644,551 | CCCAGTGACAACAAGAGATGGAGGAGA |
| TUR | 114,644,493 | 114,644,513 | CAGACAGCCAGGAATACTCAC |
| TDF | 114,622,770 | 114,622,788 | CCGCGTAGTGGAGCTGAAC |
| TDP | 114,622,738 | 114,622,764 | CTCAAGCGCAGATGAAGTGGTGGTACC |
| TDR | 114,622,698 | 114,622,716 | CTGGTGGCCGTCACAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TCATGCAGCCCATATCTGTC |
| SD | GAAGACCCACACTAGCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
