| Gene | Ipo8 |
| Chromosome | 6 |
| Deletion Start | 148,831,244 |
| Deletion End | 148,799,733 |
| Deletion Size | 31,512 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,830,849 | 148,830,868 | CAGGTGTTGCTGTCGGAAAC |
| TUP | 148,830,823 | 148,830,842 | CCTCGCCTGCTTCTGGCGGT |
| TUR | 148,830,800 | 148,830,820 | ACTGTGCCATGATCCACACTG |
| TDF | 148,799,823 | 148,799,845 | GCTCAACCTCAGAAATGCAGTAG |
| TDP | 148,799,792 | 148,799,818 | AGCCAAGAAGAGCCTGATTGAAGACGA |
| TDR | 148,799,764 | 148,799,783 | GGCAGCTTCCACCTTGACAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTGAGACGAGCATCATGG |
| SD | CAGTCAGAGAGCAACAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14312A-B6 | VGB6 | C57BL/6NTac | |
| 14312A-C10 | VGB6 | C57BL/6NTac | |
| 14312A-C12 | VGB6 | C57BL/6NTac | |
| 14312A-E4 | VGB6 | C57BL/6NTac | |
| 14312A-E6 | VGB6 | C57BL/6NTac | |
| 14312A-F5 | VGB6 | C57BL/6NTac | |
