14317 Gdf10 Status: ES cell colonies screened / QC no positives
| Gene | Gdf10 |
| Chromosome | 14 |
| Deletion Start | 33,923,899 |
| Deletion End | 33,934,532 |
| Deletion Size | 10,634 |
| Genome Build | 38 |
|
| MGI ID | MGI:95684 |
| NCBI Gene ID | 0 |
| BAC ID | 310H2 |
| BAC Library | bMQ |
| Cassette | ZEN-Ub1 |
| Clones Available | No
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,924,194 | 33,924,213 | CCCGGCTGGGTAAGTAGAAG |
| TUP | 33,924,215 | 33,924,240 | TGCACCGGGAGTCCTAATCCATCAGT |
| TUR | 33,924,259 | 33,924,280 | GAGAAGAAAGCTGCGAAGGAAC |
| TDF | 33,934,362 | 33,934,381 | TCAGAGCTGTGGGCATTGTC |
| TDP | 33,934,382 | 33,934,403 | CCTGGCATCCCAGAGCCATGCT |
| TDR | 33,934,412 | 33,934,432 | TCCAAGGGAGTTCATCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | ACAGACCGTCTCTTGGCG |
| SD | GAGAGGACAAATGCCTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
