14320 Fam176a Status: ES cell colonies screened / QC no positives
| Gene | Fam176a |
| Chromosome | 6 |
| Deletion Start | 82,071,146 |
| Deletion End | 82,092,301 |
| Deletion Size | 21,156 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,071,181 | 82,071,199 | CACGGAGCCCTTGGGAATG |
| TUP | 82,071,202 | 82,071,224 | TCTGCTCAGCAGCATCCTGGCAG |
| TUR | 82,071,228 | 82,071,250 | GCACCCACCTGAGATATAAGACC |
| TDF | 82,092,079 | 82,092,098 | AGGAGCGCGAACGAATCATC |
| TDP | 82,092,105 | 82,092,127 | ATCTGGATGAACGGGCAGCCTGA |
| TDR | 82,092,154 | 82,092,176 | GCACACTGAGCCCTAGTAGTATC |
PCR Primers
| Primer | Sequence |
| SU | TGAGGGAGGACTGTAGTGGG |
| SD | ACCATTTCCTCTTCACGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
