14321 Ppargc1a Status: ES cell colonies screened / QC no positives
| Gene | Ppargc1a |
| Chromosome | 5 |
| Deletion Start | 51,553,779 |
| Deletion End | 51,548,516 |
| Deletion Size | 5,264 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,553,509 | 51,553,531 | GCTTAGCTGCTGTCATCAGTATC |
| TUP | 51,553,471 | 51,553,498 | AGAAACTCTTTCCATGTGCCTTTGGCTC |
| TUR | 51,553,384 | 51,553,402 | CAAGCAGGCCACTGAACAC |
| TDF | 51,548,581 | 51,548,601 | GACAGCTTTCTGGGTGGATTG |
| TDP | 51,548,548 | 51,548,577 | TGGTGTAGCGACCAATCGGAAATCATATCC |
| TDR | 51,548,520 | 51,548,539 | TGTTCGCAGGCTCATTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGGCTCCGGTTTAGAGTTG |
| SD | CACTCTGTAGTCCTTAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
