14322 Fzd6 Status: ES cell colonies screened / QC no positives
| Gene | Fzd6 |
| Chromosome | 15 |
| Deletion Start | 39,007,390 |
| Deletion End | 39,036,590 |
| Deletion Size | 29,201 |
| Genome Build | 38 |
|
| MGI ID | MGI:108474 |
| NCBI Gene ID | 0 |
| BAC ID | 312C9 |
| BAC Library | bMQ |
| Cassette | ZEN-Ub1 |
| Clones Available | No
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 39,008,271 | 39,008,292 | GTGACACTAACAATCGCCTCAG |
| TUP | 39,008,294 | 39,008,323 | CGCCTCAGTTACCTGACTCTGGTTTAGAGA |
| TUR | 39,008,352 | 39,008,372 | GTCTCTACAGGCAGCTCTTTC |
| TDF | 39,036,405 | 39,036,426 | GTTCCTGAGACTGGCCTGATAG |
| TDP | 39,036,431 | 39,036,452 | CAGCACTTCACAGGCCGCCAGT |
| TDR | 39,036,471 | 39,036,491 | CAGAGATGTGGAGCCCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CCTAGCATGACATTTGACTTGG |
| SD | TGAAATCTGGGCTGTTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
