14324 Vangl2 Status: ES cell colonies screened / QC no positives
| Gene | Vangl2 |
| Chromosome | 1 |
| Deletion Start | 172,013,150 |
| Deletion End | 172,004,601 |
| Deletion Size | 8,550 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,012,340 | 172,012,362 | CAGAGGTGGTTTAGCTGAGTGAG |
| TUP | 172,012,309 | 172,012,336 | AAATTTCCCTGCTGTTCCGAAGCTCCTG |
| TUR | 172,012,284 | 172,012,301 | AGCCTGCGCCAACCTTAC |
| TDF | 172,004,812 | 172,004,832 | CCCACCATCCAGTACCACAAG |
| TDP | 172,004,787 | 172,004,810 | AACGTTGGCTGGCCAAACAGTGGA |
| TDR | 172,004,697 | 172,004,718 | TGCTCACTACCAAGCTGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTTTGTGTCCTGTCCTATGG |
| SD | TAAGTTGGAGCTCAGGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
