14331 BC029214 Status: Cancelled
| Gene | BC029214 |
| Chromosome | 2 |
| Deletion Start | 25,460,908 |
| Deletion End | 25,459,658 |
| Deletion Size | 1,251 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,460,555 | 25,460,573 | TCCAGGGTAGGGCAGTTTG |
| TUP | 25,460,530 | 25,460,551 | TGTCCAGTGGGTCCAGGGTCCT |
| TUR | 25,460,485 | 25,460,505 | ATCGGGCTTTCTGCTGGGAAC |
| TDF | 25,459,960 | 25,459,979 | GGCTGCTCTTTCTCTGGAAC |
| TDP | 25,459,936 | 25,459,959 | TGCCTCTCCAGATGTCTCATGGGA |
| TDR | 25,459,872 | 25,459,895 | AGGATCAGGGAAATCTTGAACTCC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGACCGGCATTGAAGAG |
| SD | TACCAGACCAGGACGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
