| Gene | Pibf1 |
| Chromosome | 14 |
| Deletion Start | 99,101,053 |
| Deletion End | 99,137,218 |
| Deletion Size | 36,166 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,101,556 | 99,101,578 | TGCCACACTTCATACTCCTTGTC |
| TUP | 99,101,582 | 99,101,609 | TGTTGCCTTTCTGCAGTTTGCGTATTGA |
| TUR | 99,101,631 | 99,101,654 | TGAGCCTTCAAAGGTAACTCACTC |
| TDF | 99,136,814 | 99,136,836 | GCACACCTTTAATCCTAGCATCC |
| TDP | 99,136,842 | 99,136,868 | AGGCCTATCTCTGAGAGCACGGTCAGC |
| TDR | 99,136,897 | 99,136,916 | GTGTAGCCCTGTAGCCCTTG |
PCR Primers
| Primer | Sequence |
| SU | CCTTCAAGTAAGATTCTTGCC |
| SD | TTTGTTGTTACTGGAAGACCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14340A-A3 | VGB6 | C57BL/6NTac | |
| 14340A-B9 | VGB6 | C57BL/6NTac | |
| 14340A-D6 | VGB6 | C57BL/6NTac | |
