| Gene | Polr2a |
| Chromosome | 11 |
| Deletion Start | 69,758,220 |
| Deletion End | 69,734,389 |
| Deletion Size | 23,832 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCCAGGACTTAAACAAGCGAAATG |
| TUP | | | ATGCCTGAGATGAGTGCTTGACTGGA |
| TUR | | | CTCGTTGAACCGCAGCAATG |
| TDF | | | CCAAGGGCTCCACCTACTC |
| TDP | | | CACTTCTCCTGGCTACTCACCCACTAGC |
| TDR | | | TTCTCCTCATCGCTGTCATCTG |
PCR Primers
| Primer | Sequence |
| SU | TAACCTCTGCCGTTCAGGAT |
| SD | GGTGTATGACCCAGAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14343A-F12 | VGB6 | C57BL/6NTac | |
| 14343B-E5 | VGB6 | C57BL/6NTac | |
| 14343C-A4 | VGB6 | C57BL/6NTac | |
| 14343C-B5 | VGB6 | C57BL/6NTac | |
