14350 Zic1 Status: Cancelled
| Gene | Zic1 |
| Chromosome | 9 |
| Deletion Start | 91,365,014 |
| Deletion End | 91,361,543 |
| Deletion Size | 3,472 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,364,394 | 91,364,413 | TGAACATGGCTGCACATCAC |
| TUP | 91,364,364 | 91,364,388 | TGGAGCCTTCTTCCGCTATATGCGC |
| TUR | 91,364,320 | 91,364,340 | TCCGGCTCGATCCATTTACAG |
| TDF | 91,362,186 | 91,362,204 | AGAGCTGCTGCGGTGAACG |
| TDP | 91,362,164 | 91,362,185 | CGGCCATCGCTCCTGGTAGTCA |
| TDR | 91,362,089 | 91,362,108 | GTTTGCCTGCCCACTTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGATTCGAGGCCCTCTC |
| SD | ATCCAACAAGAAATTGGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
