14366 Tmem238 Status: Cancelled
| Gene | Tmem238 |
| Chromosome | 7 |
| Deletion Start | 4,789,539 |
| Deletion End | 4,788,921 |
| Deletion Size | 619 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,789,300 | 4,789,317 | GCGCTGCTGGTGTTCTTG |
| TUP | 4,789,272 | 4,789,295 | TGCTGGGCTGGATTCTCTGGTACA |
| TUR | 4,789,230 | 4,789,248 | AGTCACGCTCCAGCTCCTG |
| TDF | 4,789,111 | 4,789,129 | CTCAGCACGCAGAGCGAAC |
| TDP | 4,789,083 | 4,789,102 | CCAGCCCTCCTCCTCCGGCT |
| TDR | 4,788,981 | 4,789,004 | TCTCATTCTTTCTGGTGTCCTCAC |
PCR Primers
| Primer | Sequence |
| SU | ATCTAGCTACATTATGCCACC |
| SD | AGAGAAAGGGAAAGGAACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 149 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
