14371 2010003K11Rik Status: Cancelled
| Gene | 2010003K11Rik |
| Chromosome | 19 |
| Deletion Start | 4,498,516 |
| Deletion End | 4,497,516 |
| Deletion Size | 1,001 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,498,174 | 4,498,194 | CGGGCAGCAAAGGGTTAAGAG |
| TUP | 4,498,147 | 4,498,172 | ACAGCTGACCTGAGGAAAGCCAAGCA |
| TUR | 4,498,055 | 4,498,077 | CCTCCTCCAACCTTCAGTGAATC |
| TDF | 4,497,667 | 4,497,684 | ACCTGGTGGCTGTGAGTG |
| TDP | 4,497,634 | 4,497,663 | CACCACTTGAAGCCACTGCTATGATGTTTC |
| TDR | 4,497,609 | 4,497,631 | CACATGCTGGAAGACCAAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GAGATGAGTGGTACGGCCAG |
| SD | CAGCCTTTCTTTGGCTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 140 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
