| Gene | Scx |
| Chromosome | 15 |
| Deletion Start | 76,457,605 |
| Deletion End | 76,459,176 |
| Deletion Size | 1,572 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,458,267 | 76,458,289 | GCAGCATTCCCTAGCATCTACAC |
| TUP | 76,458,291 | 76,458,314 | TGCCAACAAAGCTGTGCCCAAAGG |
| TUR | 76,458,326 | 76,458,345 | CCCAAGGTATGGCTCCTCTG |
| TDF | 76,458,408 | 76,458,426 | GAGATGGGCCACCCAGAAG |
| TDP | 76,458,442 | 76,458,461 | CAGGCAGCAGGAACCTGGCA |
| TDR | 76,458,478 | 76,458,498 | GATGGGAAACGAGCCTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CCAACCAGAAAGATGCTCG |
| SD | CCTGTCCTCACTCTGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14378A-H1 | VGB6 | C57BL/6NTac | |
| 14378A-A3 | VGB6 | C57BL/6NTac | |
| 14378A-C3 | VGB6 | C57BL/6NTac | |
| 14378A-D12 | VGB6 | C57BL/6NTac | |
| 14378A-H9 | VGB6 | C57BL/6NTac | |
| 14378A-G4 | VGB6 | C57BL/6NTac | |
