| Gene | Cd93 |
| Chromosome | 2 |
| Deletion Start | 148,443,421 |
| Deletion End | 148,441,221 |
| Deletion Size | 2,201 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,442,555 | 148,442,573 | CCGGCCTGGATTTCGACTG |
| TUP | 148,442,520 | 148,442,549 | TGATCTAGTAACTTGTGCCTCCAGGAACCC |
| TUR | 148,442,493 | 148,442,511 | CCTCCTGTGCATGGGTTTG |
| TDF | 148,441,408 | 148,441,428 | CAGTCAGCAGGCCATTTGTTG |
| TDP | 148,441,384 | 148,441,406 | TTAAAGCCAACCTCAGCAGCCCA |
| TDR | 148,441,338 | 148,441,356 | GCCGCTACTCCACATGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCAGAGACTGCCCTCTGAAC |
| SD | TTGGTTTGCTGTCTGTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14382A-B1 | VGB6 | C57BL/6NTac | |
| 14382A-D5 | VGB6 | C57BL/6NTac | |
| 14382A-D1 | VGB6 | C57BL/6NTac | |
