14385 BC027072 Status: Cancelled
| Gene | BC027072 |
| Chromosome | 17 |
| Deletion Start | 71,752,677 |
| Deletion End | 71,744,414 |
| Deletion Size | 8,264 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,752,192 | 71,752,210 | CCGCCAATCGGCTACTGAG |
| TUP | 71,752,166 | 71,752,191 | TCAAAGGGCAGAGTGGATTTCCCTGA |
| TUR | 71,752,119 | 71,752,139 | TGGTGTGCAGGTAGGCATAAG |
| TDF | 71,744,931 | 71,744,953 | AACTGGCTCCTACCACGTCTAAC |
| TDP | 71,744,905 | 71,744,930 | CAGACAGGCATGCAGAACCGACAATC |
| TDR | 71,744,870 | 71,744,891 | GGCCTCTGCCTTTCCATATCAC |
PCR Primers
| Primer | Sequence |
| SU | TTAAAGCACAAGCACATGCC |
| SD | TGTGGTTTAAATGACGCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
