| Gene | 2410066E13Rik |
| Chromosome | 6 |
| Deletion Start | 54,681,795 |
| Deletion End | 54,699,153 |
| Deletion Size | 17,359 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,682,532 | 54,682,553 | CCAGGAACTACAGCGACCATAC |
| TUP | 54,682,554 | 54,682,575 | CTTTGGCCAGCATCCCTCCCAC |
| TUR | 54,682,578 | 54,682,599 | AAACTGTCAGCACCAGAATACC |
| TDF | 54,698,257 | 54,698,277 | CACCTGCTGCCAAGTCTGATG |
| TDP | 54,698,278 | 54,698,302 | ACCCTAGTTCTGCCTCTGGGCATCA |
| TDR | 54,698,328 | 54,698,347 | GGTCACAGGACAATGCACAG |
PCR Primers
| Primer | Sequence |
| SU | AGAGAAAGCCTTCCAAACTG |
| SD | CATGCTTTCTAAGCGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14387A-E2 | VGB6 | C57BL/6NTac | |
| 14387A-C8 | VGB6 | C57BL/6NTac | |
